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sol-gel-based selex  (MicroFluidic Systems)

 
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    Structured Review

    MicroFluidic Systems sol-gel-based selex
    The <t>Cell-SELEX</t> process performed on a microfluidic chip. (a) Incubation and mixing of the single-stranded DNA (ssDNA) library with target cell-magnetic bead complexes in binding buffer and fetal bovine serum (FBS) in the positive selection chamber. (b) Washing and removal of unbound ssDNA using the washing buffer and removal of wastes through the waste collection chamber. (c) Thermal release of bound ssDNA and application of a magnetic field to isolate the cells and transfer the bound ssDNA to the negative selection chamber. (d) Negative selection in the negative selection chamber with negative control cell-magnetic bead complexes in binding buffer. (e) Magnetic separation of negative control cell-magnetic bead complexes, and transfer of supernatant to the PCR chamber. (f) PCR amplification of selected sequences. The amplified product was then transferred to the positive selection chamber for the next round of SELEX. Blue arrows indicate the micro-chamber in which the respective reaction step was carried out.
    Sol Gel Based Selex, supplied by MicroFluidic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sol-gel-based+selex/sol+gel+based+selex/pmc05498186-45-5-18
    Average 90 stars, based on 1 article reviews
    sol-gel-based selex - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Automated selection of aptamers against cholangiocarcinoma cells on an integrated microfluidic platform"

    Article Title: Automated selection of aptamers against cholangiocarcinoma cells on an integrated microfluidic platform

    Journal: Biomicrofluidics

    doi: 10.1063/1.4991005

    The Cell-SELEX process performed on a microfluidic chip. (a) Incubation and mixing of the single-stranded DNA (ssDNA) library with target cell-magnetic bead complexes in binding buffer and fetal bovine serum (FBS) in the positive selection chamber. (b) Washing and removal of unbound ssDNA using the washing buffer and removal of wastes through the waste collection chamber. (c) Thermal release of bound ssDNA and application of a magnetic field to isolate the cells and transfer the bound ssDNA to the negative selection chamber. (d) Negative selection in the negative selection chamber with negative control cell-magnetic bead complexes in binding buffer. (e) Magnetic separation of negative control cell-magnetic bead complexes, and transfer of supernatant to the PCR chamber. (f) PCR amplification of selected sequences. The amplified product was then transferred to the positive selection chamber for the next round of SELEX. Blue arrows indicate the micro-chamber in which the respective reaction step was carried out.
    Figure Legend Snippet: The Cell-SELEX process performed on a microfluidic chip. (a) Incubation and mixing of the single-stranded DNA (ssDNA) library with target cell-magnetic bead complexes in binding buffer and fetal bovine serum (FBS) in the positive selection chamber. (b) Washing and removal of unbound ssDNA using the washing buffer and removal of wastes through the waste collection chamber. (c) Thermal release of bound ssDNA and application of a magnetic field to isolate the cells and transfer the bound ssDNA to the negative selection chamber. (d) Negative selection in the negative selection chamber with negative control cell-magnetic bead complexes in binding buffer. (e) Magnetic separation of negative control cell-magnetic bead complexes, and transfer of supernatant to the PCR chamber. (f) PCR amplification of selected sequences. The amplified product was then transferred to the positive selection chamber for the next round of SELEX. Blue arrows indicate the micro-chamber in which the respective reaction step was carried out.

    Techniques Used: Incubation, Binding Assay, Selection, Negative Control, Amplification

    Agarose gel electrophoresis: (a) PCR products were electrophoresed on an agarose gel (2%) and stained with ethidium bromide. For panels (a) and (b), lane L = 50-bp DNA ladder, lane N = ddH2O (negative control), lane P = 1 μM of ssDNA library, and lanes S1 to S6= PCR products (30 cycles) obtained after 1 to 6 rounds of SELEX, respectively, with HuCCT-1 (a) and SNU-478 (b) cells. (c) Gel electrophoresis analysis after a second round of negative selection performed with MMNK-1 cells and WBCs. Lanes S and H depict PCR products after negative selection with SNU-478 and HuCCT-1 cells, respectively.
    Figure Legend Snippet: Agarose gel electrophoresis: (a) PCR products were electrophoresed on an agarose gel (2%) and stained with ethidium bromide. For panels (a) and (b), lane L = 50-bp DNA ladder, lane N = ddH2O (negative control), lane P = 1 μM of ssDNA library, and lanes S1 to S6= PCR products (30 cycles) obtained after 1 to 6 rounds of SELEX, respectively, with HuCCT-1 (a) and SNU-478 (b) cells. (c) Gel electrophoresis analysis after a second round of negative selection performed with MMNK-1 cells and WBCs. Lanes S and H depict PCR products after negative selection with SNU-478 and HuCCT-1 cells, respectively.

    Techniques Used: Agarose Gel Electrophoresis, Staining, Negative Control, Nucleic Acid Electrophoresis, Selection

    Melting curves analysis of the ssDNA pools after each round of SELEX. (a) Melting curve of SELEX rounds 1 to 6 for SNU-478 cell line. (b) Melting curve of SELEX rounds 1 to 6 for HuCCT-1 cell lines. The RT-PCR products of the 5th and 6th cycles depicted a melting temperature of 84.88 °C and 85.02 °C.
    Figure Legend Snippet: Melting curves analysis of the ssDNA pools after each round of SELEX. (a) Melting curve of SELEX rounds 1 to 6 for SNU-478 cell line. (b) Melting curve of SELEX rounds 1 to 6 for HuCCT-1 cell lines. The RT-PCR products of the 5th and 6th cycles depicted a melting temperature of 84.88 °C and 85.02 °C.

    Techniques Used: Reverse Transcription Polymerase Chain Reaction

    Related Articles

    Electrophoresis:

    Article Title: Automated selection of aptamers against cholangiocarcinoma cells on an integrated microfluidic platform
    Article Snippet: Capillary electrophoresis (CE)-based SELEX, 18 sol-gel-based SELEX, 19 and magnetic bead-based SELEX 20–22 have all been integrated into microfluidic systems, resulting in the rapid and highly efficient isolation of aptamers.

    Isolation:

    Article Title: Automated selection of aptamers against cholangiocarcinoma cells on an integrated microfluidic platform
    Article Snippet: Capillary electrophoresis (CE)-based SELEX, 18 sol-gel-based SELEX, 19 and magnetic bead-based SELEX 20–22 have all been integrated into microfluidic systems, resulting in the rapid and highly efficient isolation of aptamers.

    Incubation:

    Article Title: Automated selection of aptamers against cholangiocarcinoma cells on an integrated microfluidic platform
    Article Snippet: Capillary electrophoresis (CE)-based SELEX, 18 sol-gel-based SELEX, 19 and magnetic bead-based SELEX 20–22 have all been integrated into microfluidic systems, resulting in the rapid and highly efficient isolation of aptamers.

    Binding Assay:

    Article Title: Automated selection of aptamers against cholangiocarcinoma cells on an integrated microfluidic platform
    Article Snippet: Capillary electrophoresis (CE)-based SELEX, 18 sol-gel-based SELEX, 19 and magnetic bead-based SELEX 20–22 have all been integrated into microfluidic systems, resulting in the rapid and highly efficient isolation of aptamers.

    Selection:

    Article Title: Automated selection of aptamers against cholangiocarcinoma cells on an integrated microfluidic platform
    Article Snippet: Capillary electrophoresis (CE)-based SELEX, 18 sol-gel-based SELEX, 19 and magnetic bead-based SELEX 20–22 have all been integrated into microfluidic systems, resulting in the rapid and highly efficient isolation of aptamers.

    Negative Control:

    Article Title: Automated selection of aptamers against cholangiocarcinoma cells on an integrated microfluidic platform
    Article Snippet: Capillary electrophoresis (CE)-based SELEX, 18 sol-gel-based SELEX, 19 and magnetic bead-based SELEX 20–22 have all been integrated into microfluidic systems, resulting in the rapid and highly efficient isolation of aptamers.

    Amplification:

    Article Title: Automated selection of aptamers against cholangiocarcinoma cells on an integrated microfluidic platform
    Article Snippet: Capillary electrophoresis (CE)-based SELEX, 18 sol-gel-based SELEX, 19 and magnetic bead-based SELEX 20–22 have all been integrated into microfluidic systems, resulting in the rapid and highly efficient isolation of aptamers.

    Agarose Gel Electrophoresis:

    Article Title: Automated selection of aptamers against cholangiocarcinoma cells on an integrated microfluidic platform
    Article Snippet: Capillary electrophoresis (CE)-based SELEX, 18 sol-gel-based SELEX, 19 and magnetic bead-based SELEX 20–22 have all been integrated into microfluidic systems, resulting in the rapid and highly efficient isolation of aptamers.

    Staining:

    Article Title: Automated selection of aptamers against cholangiocarcinoma cells on an integrated microfluidic platform
    Article Snippet: Capillary electrophoresis (CE)-based SELEX, 18 sol-gel-based SELEX, 19 and magnetic bead-based SELEX 20–22 have all been integrated into microfluidic systems, resulting in the rapid and highly efficient isolation of aptamers.

    Nucleic Acid Electrophoresis:

    Article Title: Automated selection of aptamers against cholangiocarcinoma cells on an integrated microfluidic platform
    Article Snippet: Capillary electrophoresis (CE)-based SELEX, 18 sol-gel-based SELEX, 19 and magnetic bead-based SELEX 20–22 have all been integrated into microfluidic systems, resulting in the rapid and highly efficient isolation of aptamers.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Automated selection of aptamers against cholangiocarcinoma cells on an integrated microfluidic platform
    Article Snippet: Capillary electrophoresis (CE)-based SELEX, 18 sol-gel-based SELEX, 19 and magnetic bead-based SELEX 20–22 have all been integrated into microfluidic systems, resulting in the rapid and highly efficient isolation of aptamers.



    Similar Products

    sol-gel-based selex  (MicroFluidic Systems)
    90
    MicroFluidic Systems sol-gel-based selex
    The <t>Cell-SELEX</t> process performed on a microfluidic chip. (a) Incubation and mixing of the single-stranded DNA (ssDNA) library with target cell-magnetic bead complexes in binding buffer and fetal bovine serum (FBS) in the positive selection chamber. (b) Washing and removal of unbound ssDNA using the washing buffer and removal of wastes through the waste collection chamber. (c) Thermal release of bound ssDNA and application of a magnetic field to isolate the cells and transfer the bound ssDNA to the negative selection chamber. (d) Negative selection in the negative selection chamber with negative control cell-magnetic bead complexes in binding buffer. (e) Magnetic separation of negative control cell-magnetic bead complexes, and transfer of supernatant to the PCR chamber. (f) PCR amplification of selected sequences. The amplified product was then transferred to the positive selection chamber for the next round of SELEX. Blue arrows indicate the micro-chamber in which the respective reaction step was carried out.
    Sol Gel Based Selex, supplied by MicroFluidic Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sol-gel-based+selex/sol+gel+based+selex/pmc05498186-45-5-18
    Average 90 stars, based on 1 article reviews
    sol-gel-based selex - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    The Cell-SELEX process performed on a microfluidic chip. (a) Incubation and mixing of the single-stranded DNA (ssDNA) library with target cell-magnetic bead complexes in binding buffer and fetal bovine serum (FBS) in the positive selection chamber. (b) Washing and removal of unbound ssDNA using the washing buffer and removal of wastes through the waste collection chamber. (c) Thermal release of bound ssDNA and application of a magnetic field to isolate the cells and transfer the bound ssDNA to the negative selection chamber. (d) Negative selection in the negative selection chamber with negative control cell-magnetic bead complexes in binding buffer. (e) Magnetic separation of negative control cell-magnetic bead complexes, and transfer of supernatant to the PCR chamber. (f) PCR amplification of selected sequences. The amplified product was then transferred to the positive selection chamber for the next round of SELEX. Blue arrows indicate the micro-chamber in which the respective reaction step was carried out.

    Journal: Biomicrofluidics

    Article Title: Automated selection of aptamers against cholangiocarcinoma cells on an integrated microfluidic platform

    doi: 10.1063/1.4991005

    Figure Lengend Snippet: The Cell-SELEX process performed on a microfluidic chip. (a) Incubation and mixing of the single-stranded DNA (ssDNA) library with target cell-magnetic bead complexes in binding buffer and fetal bovine serum (FBS) in the positive selection chamber. (b) Washing and removal of unbound ssDNA using the washing buffer and removal of wastes through the waste collection chamber. (c) Thermal release of bound ssDNA and application of a magnetic field to isolate the cells and transfer the bound ssDNA to the negative selection chamber. (d) Negative selection in the negative selection chamber with negative control cell-magnetic bead complexes in binding buffer. (e) Magnetic separation of negative control cell-magnetic bead complexes, and transfer of supernatant to the PCR chamber. (f) PCR amplification of selected sequences. The amplified product was then transferred to the positive selection chamber for the next round of SELEX. Blue arrows indicate the micro-chamber in which the respective reaction step was carried out.

    Article Snippet: Capillary electrophoresis (CE)-based SELEX, 18 sol-gel-based SELEX, 19 and magnetic bead-based SELEX 20–22 have all been integrated into microfluidic systems, resulting in the rapid and highly efficient isolation of aptamers.

    Techniques: Incubation, Binding Assay, Selection, Negative Control, Amplification

    Agarose gel electrophoresis: (a) PCR products were electrophoresed on an agarose gel (2%) and stained with ethidium bromide. For panels (a) and (b), lane L = 50-bp DNA ladder, lane N = ddH2O (negative control), lane P = 1 μM of ssDNA library, and lanes S1 to S6= PCR products (30 cycles) obtained after 1 to 6 rounds of SELEX, respectively, with HuCCT-1 (a) and SNU-478 (b) cells. (c) Gel electrophoresis analysis after a second round of negative selection performed with MMNK-1 cells and WBCs. Lanes S and H depict PCR products after negative selection with SNU-478 and HuCCT-1 cells, respectively.

    Journal: Biomicrofluidics

    Article Title: Automated selection of aptamers against cholangiocarcinoma cells on an integrated microfluidic platform

    doi: 10.1063/1.4991005

    Figure Lengend Snippet: Agarose gel electrophoresis: (a) PCR products were electrophoresed on an agarose gel (2%) and stained with ethidium bromide. For panels (a) and (b), lane L = 50-bp DNA ladder, lane N = ddH2O (negative control), lane P = 1 μM of ssDNA library, and lanes S1 to S6= PCR products (30 cycles) obtained after 1 to 6 rounds of SELEX, respectively, with HuCCT-1 (a) and SNU-478 (b) cells. (c) Gel electrophoresis analysis after a second round of negative selection performed with MMNK-1 cells and WBCs. Lanes S and H depict PCR products after negative selection with SNU-478 and HuCCT-1 cells, respectively.

    Article Snippet: Capillary electrophoresis (CE)-based SELEX, 18 sol-gel-based SELEX, 19 and magnetic bead-based SELEX 20–22 have all been integrated into microfluidic systems, resulting in the rapid and highly efficient isolation of aptamers.

    Techniques: Agarose Gel Electrophoresis, Staining, Negative Control, Nucleic Acid Electrophoresis, Selection

    Melting curves analysis of the ssDNA pools after each round of SELEX. (a) Melting curve of SELEX rounds 1 to 6 for SNU-478 cell line. (b) Melting curve of SELEX rounds 1 to 6 for HuCCT-1 cell lines. The RT-PCR products of the 5th and 6th cycles depicted a melting temperature of 84.88 °C and 85.02 °C.

    Journal: Biomicrofluidics

    Article Title: Automated selection of aptamers against cholangiocarcinoma cells on an integrated microfluidic platform

    doi: 10.1063/1.4991005

    Figure Lengend Snippet: Melting curves analysis of the ssDNA pools after each round of SELEX. (a) Melting curve of SELEX rounds 1 to 6 for SNU-478 cell line. (b) Melting curve of SELEX rounds 1 to 6 for HuCCT-1 cell lines. The RT-PCR products of the 5th and 6th cycles depicted a melting temperature of 84.88 °C and 85.02 °C.

    Article Snippet: Capillary electrophoresis (CE)-based SELEX, 18 sol-gel-based SELEX, 19 and magnetic bead-based SELEX 20–22 have all been integrated into microfluidic systems, resulting in the rapid and highly efficient isolation of aptamers.

    Techniques: Reverse Transcription Polymerase Chain Reaction